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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical function in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two added splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind precisely to your focus on antibody and block antibody binding. These peptide typically incorporates the epitope identified from the antibody.
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This manual outlines further probable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to be sure the very best results are...
Antibodies bound to the blocking peptide no longer bind to your epitope within the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody by itself, you can see which staining is particular.
Focus on Information Cyclin E belongs to the hugely conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity more info is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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